Fig. 3

YS110 alters expressions and phosphorylation of cell cycle regulators. a At 24 h after treatment with 2 μg/mL of YS110, p21 protein expression increased while cyclinB1 protein expression decreased. Inhibitory phosphorylation of cdc2 Tyr15 and cdc25C Ser216 were elevated while there was no significant change in whole cdc2 and cdc25C protein. β-actin was used as an internal control. b Alteration in subcellular distribution of whole cdc25C by YS110 treatment is shown. At 24 h after treatment with 2 μg/mL of YS110, nuclear cdc25C protein decreased while cytoplasmic cdc25C protein increased. LaminA/C was used as a nuclear marker and Na–K ATPase was used as a cytoplasmic marker. The bar graph demonstrates the densitometric analysis of a Western blot of whole cdc25C. Results are normalized by the densitometry of control cells. All experiments were performed in triplicate and a representative experiment is shown