Fig. 6
Effective inhibition of mesothelioma cell growth in vivo. A Combination effects of YS110 and PMX were examined using xenograft models with JMN cells transplantation subcutaneously. The weight of tumors treated with YS110 was insignificantly reduced but PMX treatment induced a significantly reduced tumor weight (*p < 0.05). A combination of YS110 and PMX treatment reduced tumor weight compared with the weight of tumors with YS110 single treatment and PMX single treatment synergistically (**p < 0.05). B Combination effects of tumor growth in vivo were examined using a measurement of MIB-1 index histologically. MIB-1 index was significantly reduced with YS110 or PMX single treatment compared with control IgG treatment (*p < 0.05). The MIB-1 index after combinatory treatment with YS110 and PMX was significantly reduced compared with YS110 or PMX single treatment (**p < 0.05). C Sarcomatous mesothelioma is shown in HE staining (a) and stained with anti-CD26 polyclonal antibody (R&D) (b) MIB-1 index was measured by immunohistochemistry using the anti-Ki-67 monoclonal antibody (DAKO, clone MIB-1). Staining of Ki-67 antigens in nucleus is seen in tumors treated with control IgG (c), YS110 (d), and both YS110 and PMX (e). The number of Ki-67 positive nucleus was reduced after combinatory treatment of YS110 and PMX and the necrotic area is indicated by an asterisk (e)