Fig. 1

The TMZ-treated U251TMZ1 and U251TMZ2 have an increased CIN and ploidy. a Representative karyograms of U251 and U251TMZ1. The structurally abnormal chromosomes are marked. b Distribution of chromosomes across 200 metaphases of U251 and U251TMZ2 cell lines. The insert table shows a percentage of metaphases with numbers of chromosomes >60 or >90. c The karyotype differences between cell lines were demonstrated by alignment and comparison of karyographs of vehicle- and TMZ-treated derivatives. A list of all CCAs/NCCAs (in the same order as depicted on the x-axis of karyographs) and their copy number variation can be found in Additional file 1: Table S1. d A summary of karyotypic parameters of each cell line. e Chromosomal ideograms showing the areas of genetic gain/loss. Bars on the left (red colour) represent areas of copy number loss, whereas bars on the right (green colour) represent areas of copy number gain. Detailed description of copy number alterations (CNAs) can be found in Additional file 2: Table S2