Fig. 3
Tumorigenicity and metastatic potential of HCC40-CL cells in NOD/SCID mice. a NOD/SCID mice inoculated subcutaneously with 1 × 106 HCC40-CL cells at 8 weeks post-injection. The middle panel showed the corresponding xenograft tumor derived from HCC40-CL cells (scale bar 10 mm) and H&E staining of the paraffin-embedded tissue section (Magnification: 200×, 400×). The right panel showed the growth curve of HCC40-CL cell-derived xenografts (n = 4). b Protein expression of E-cadherin in HCC21 (negative control), Hep3B (positive control), adjacent non-tumor liver tissue and tumor specimen from patient HCC40, and HCC40-CL cells. β-actin served as loading control. c HCC40-CL cells were injected intravenously into NOD/SCID mice. H&E staining showed extensive metastases observed in the liver, kidney, lung, heart, spleen and pancreas of the mice