Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: RETRACTED ARTICLE: Long noncoding RNA ANRIL is activated by hypoxia-inducible factor-1α and promotes osteosarcoma cell invasion and suppresses cell apoptosis upon hypoxia

Fig. 2

HIF-1α binds to hypoxia responsive elements of the ANRIL promoter under hypoxic conditions. a Schematic representation of the putative binding sites of hypoxia responsive elements (HREs) in the ANRIL promoter. b HIF-1α mRNA levels under both normoxic and hypoxic conditions (24 h) were analyzed by real-time PCR. β-actin was used as the internal control. c HIF-1α protein levels under both normoxic and hypoxic conditions were analyzed by Western blotting. β-actin was used as the internal control (**, P < 0.01, n = 3). d EMSA showed the interaction of HIF-1α with the ANRIL promoter in vitro. e ChIP showed the interaction of HIF- 1α with the ANRIL promoter. The HIF-1α antibody effectively enriched the DNA sequence covering the putative binding element. Normal rabbit IgG was used as a negative control, and an anti-HIF-1α polymerase antibody was used as a positive control. Sonicated DNA fragments were used as input

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us