Fig. 8

Kallistatin induces NCI-H446 cells apoptosis. a The cells were collected and stained with annexin V-FITC/PI (1: PBS; 2: 50 µg/ml kallistatin; 3: 100 µg/ml kallistatin; 4: 200 µg/ml kallistatin); b the quantitative results of flow cytometry analysis. *P < 0.05, N = 3. c Immunoblotting were performed using 200 μg total proteins from each sample. Relative levels of Bcl-2 and pro-caspase 9 were decreased, while Bax, cleaved caspase 9 and cleaved caspase-3 were increased. The protein levels were normalized with β-actin. d, e The relative Bcl-2, Bax levels were calculated and plotted. The data are expressed as mean ± SD from three independent experiments (N = 3 each). *P < 0.05