Fig. 5
From: Screening of breast cancer stem cell inhibitors using a protein kinase inhibitor library
Effects of protein kinase inhibitors on migration, colony formation ability and TGF-β1-induced EMT in MCF-7 cells. a, b The inhibitors of CAMKII, JAK-3 and IKK decrease cell migration (a) and colony formation (b) in MCF-7 cells. c TGF-β1 (10 ng/ml) induces morphological changes in MCF-7 cells, including a loss of cell contact and elongation, which are ameliorated by CAMKII, JAK-3 and IKK inhibitors (10, 10, 5 µM, respectively). Representative photos of cell morphology are shown (×100 magnification). d, e Western blot analysis with a quantitation bar demonstrates TGF-β1-induced EMT as a decrease in an epithelial cell marker, cytokeratin 18 (CK18), and an increase in a mesenchymal marker, N-cadherin (N-cad). TGF-β1-induced EMT is ameliorated by CAMKII, JAK-3 and IKK inhibitors. β-actin is used as a loading control. The data shown represent the mean ± SD of 5 independent experiments. *p < 0.05 vs. control, #p < 0.05 vs. others