Fig. 1
From: PI3Kα isoform-dependent activation of RhoA regulates Wnt5a-induced osteosarcoma cell migration
Wnt5a triggers RhoA activation of osteosarcoma cells. (a and b) Human osteosarcoma cells MG-63 (a) and U20S (b), serum-deprived for 24 h, were untreated or treated with 100 ng/mL of Wnt5a and harvested at 30 min after the start of treatment for small G-protein activation assays. Data were presented as mean ± SD of 3 determinations. The relative Rho activity was normalized to the average value of Wnt5a-untreated group. c Serum-deprived MG-63 cells were pre-treated with 1 µg/mL sfrp2 (an antagonist that directly binds to Wnt5a) for 1 h, then were incubated with 100 ng/mL Wnt5a and harvested at 30 min after the start of Wnt5a treatment. Data were presented as mean ± SD of 3 determinations. The relative RhoA activity was normalized to the average value of sfrp2-untreated group