Fig. 3

miR-484 suppresses the migration and invasion of CC cells and down-regulates the EMT process. a, b After transfection 48 h, cell migration (a) and invasion (b) were evaluated using a transwell system with 8 μm pores in polycarbonate membranes. Representative views of migratory or invasive cells on the membrane were presented below. All pictures were photographed at ×20 magnification. c Protein levels of EMT-associated markers were assessed by western blotting after transfection 48 h. d RT-qPCR analysis for the expression of EMT transcription factors ZEB1, Snail, Slug and Twist2 in HeLa cells transfected with miR-484 or the control vector. The control was normalized to 1. All data represent mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001