Fig. 8

Knockdown of SMAD2/ZEB1 abrogates the effects induced by ASO-miR-484. a After co-transfection with ASO-miR-484 and pshR-SMAD2/ZEB1 or the control vector for 48 h, MTT assay was performed to detect cell viability in CC cells. b The transfected cells were submitted to colony formation assays to test the proliferation of CC cells. c The expression of EMT markers (E-cadherin and vimentin) was detected by western blot analysis. All data represent mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001