Table 1 The oligonucleotides and primers used in this study
Name | Sequence (5′–3′) |
|---|---|
Pri-miR-484-forward | CGACGGATCCAAGCGCACCCTTCACTTC |
Pri-miR-484-reverse | GCTCGAATTCCGCTTCAAGGTTCCTTTCG |
ASO-miR-484 | AUCGGGAGGGGACUGAGCCUGA |
ASO-NC | CAGUACUUUUGUGUAGUACAA |
SMAD2-forward | GCGGAATTCAACATGTCGTCCATCTTGCCATTC |
SMAD2-reverse | CCAGCTCGAGTTATGACATGCTTGAGCAACG |
ZEB1-forward | GCGGATCCGCGGATGGCCCCAGGTGTAAG |
ZEB1-reverse | GACGCCTCGAGTTAGGCTTCATTTGTCTTTTCTTC |
SMAD2-3′UTR-S | GATCCTTTCTAGTATCTTACAGCCTGATAAGCTTG |
SMAD2-3′UTR-AS | AATTCAAGCTTATCAGGCTGTAAGATACTAGAAAG |
ZEB1-3′UTR-S | GATCCTTCTGGAGAGGTCAGAGTTGACAAGCTTG |
ZEB1-3′UTR-AS | AATTCAAGCTTGTCAACTCTGACCTCTCCAGAAG |
SMAD2-3′UTRmut-S | GATCCTTTCTAGTATCTTACCGAGCTATAAGCTTG |
SMAD2-3′UTRmut-AS | AATTCAAGCTTATAGCTCGGTAAGATACTAGAAAG |
ZEB1-3′UTRmut-S | GATCCTTCTGGAGAGGTCATCTAACTACAAGCTTG |
ZEB1-3′UTRmut-AS | AATTCAAGCTTGTAGTTAGATGACCTCTCCAGAAG |
miR-484-RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACATCGGGAG |
miR-484-forward | TGCAGTCAGGCTCAGTCCCC |
U6-RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATATGGAAC |
U6-forward | TGCGGGTGCTCGCTTCGGCAGC |
Reverse primer | CCAGTGCAGGGTCCGAGGT |
qPCR-actin-forward | CGTGACATTAAGGAGAAGCTG |
qPCR-actin-reverse | CTAGAAGCATTTGCGGTGGAC |
qPCR-ZEB1-forward | TAAAGTGGCGGTAGATGGTA |
qPCR-ZEB1-reverse | ACTGTTTGTAGCGACTGGATT |
qPCR-FN1-forward | CAGTGGGAGACCTCGAGAAG |
qPCR-FN1-reverse | TCCCTCGGAACATCAGAAAC |
qPCR-Snail-forward | TTCTCTAGGCCCTGGCTGC |
qPCR-Snail-reverse | TACTTCTTGACATCTGAGTGGGTCTG |
qPCR-Twist-forward | GCAAGAAGTCGAGCGAAGAT |
qPCR-Twist-reverse | GCTCTGCAGCTCCTCGAA |