Fig. 2

MALAT1 directly targeted miR-1297 in GC cells. a Bioinformatic analysis identified a potential miR-1297 target site of MALAT1 by software starbase (http://starbase.sysu.edu.cn/). b The miR-1297 expression levels in 78 pairs of gastric cancer and corresponding non-cancerous gastric tissues was detected by qRT-PCR assays. **P < 0.05, the internal control was U6, Student’s t test. c The miR-1297 expression in gastric cancer cells and an immortalized normal gastric epithelial cell line GES-1 was detected by qRT-PCR assays, the internal control was U6, **P < 0.05. d Pearson correlation analysis was used to detect the significance of association between MALAT1 and miR-1297 expression. e The miR-1297 expression in MKN45 and MKN28 cells was detected after MALAT1 silencing by qRT-PCR assays, the internal control was U6, **P < 0.05. f The MALAT1 expression in MKN45 or MKN28 cells was detected after transfecting miR-1297 by qRT-PCR assays, the internal control was GAPDH, **P < 0.05. g Co-transfection of miR-1297 mimic and PMIRGLO-MALAT1-WT strongly decreased the luciferase activity, while co-transfection of miR-1297 mimic and PMIRGLO-MALAT1-MUT and NC group did not change the luciferase activity, h Co-transfection of PmiRGLO-miR-1297-WT and MALAT1 overexpressed plasmid strongly decreased the luciferase activity, while co-transfection of PMIRGLO-miR-1297-MUT and MALAT1 overexpressed plasmid did not change the luciferase activity, Results were represented as the average ± SD based on 3 independent experiments. **P < 0.05