Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: Hypoxia and TGF-β1 induced PLOD2 expression improve the migration and invasion of cervical cancer cells by promoting epithelial-to-mesenchymal transition (EMT) and focal adhesion formation

Fig. 2

Minoxidil-mediated inhibition of PLOD2 attenuates cell migration and invasion. a Inhibition of PLOD2 by minoxidil (0.5 mM) in HeLa and SiHa cells. Cells were treated with minoxidil (0.5 mM) for 24, 48 or 72 h and then subjected to a Western blot analysis; α-tubulin was used as a loading control. b, d The inhibition of PLOD2 invasion and migration. Cells treated with minoxidil (0.5 mM) for 48 h and control cells were subjected to transwell migration (b) and matrigel-coated invasion assays (d). Photos were captured under a ×200 objective lens. c, e Statistical analysis of migration (c) and invasion (e) based on the mean ± SD of at least three independent experiments. p values were obtained using Student’s t test. p < 0.05 is indicated by “**”, and p < 0.001 is indicated by “***”. f Scratch assays further supported that minoxidil (0.5 mM) treatment inhibited the migration of HeLa and SiHa cells. Photos were captured under a ×200 objective lens. g Statistical data were obtained as described above

Back to article page
\