Fig. 3

The construction of over expression and interference models. a qRT-PCR analysis indicated that COL1A1 expression levels were increased significantly in CaSki and Hela cells with COL1A1 activation plasmids, while COL1A1 expression levels were decreased significantly in CaSki and Hela cells with COL1A1 shRNA plasmids. b Protein levels indicated the COL1A1-shRNA and COL1A1 Activation cell models were constructed successfully. *P < 0.05 vs control. Con, cells without plasmid. COL1A1 Activation, cells transfected with COL1A1 Activation plasmids. Activation NC, cells transfected with scramble control of activation plasmid. COL1A1 shRNA, cells transfected with COL1A1 shRNA plasmid. shRNA NC, cells transfected scramble control of shRNA plasmid