Fig. 2
From: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells
CRISPR/Cas9 genome editing of the ARNT gene promoter. a Location of CRISPR/Cas9 target sequences (light grey) within Region 3. HBS HIF binding site, HAS HIF ancillary sequence, PAM protospacer-adjacent motif. b Cleavage detection assay of untransfected (UT), CRISPR/Cas9-Target 1 (T1) or CRISPR/Cas9-Target 2 (T2) transfected Hep3B cells. Fragment sizes are given in bp. The arrow indicates the re-hybridised PCR product. Representative result of n = 3 independent experiments. c. p. cleavage products