Fig. 5
From: ARNT is a potential direct HIF-1 target gene in human Hep3B hepatocellular carcinoma cells
Effects of genome editing on HIF target gene expression. a VEGFA mRNA expression in CRISPR/Cas9 genome edited Hep3B cells cultured in Lumox® gas permeable petri-dishes (corresponding to Fig. 4). Values are presented as mean ± SEM of n = 3 independent experiments. b Reporter gene assay. Ratios of Firefly/Renilla luciferase activity were measured in normoxic/hypoxic Hep3B cells after 8 h and normalised to normoxic control cells (Ctrl. (N)). Values are presented as mean ± SEM of n = 3 independent experiments. N normoxia, H hypoxia