Fig. 6

Role of ERK1/2 activation in h-TGF-β1 anti-proliferative activity of KKU-M213 cells. Cells were treated with 5 ng/mL h-TGF-β1 with or without 1 µM U0126 in 10% FBS media for indicated times and the numbers of viable cells were quantified using MTT assay (a). The graphs are presented as mean ± SEM from three independent experiments. *P < 0.05 and **P < 0.001 compared to control at 72 h. For the colony formation, 200 cells treated as those in MTT assay were incubated for 7 days and colony formation ability was quantified as percent ± SEM of crystal violet-stained area compared to control (b). ** P < 0.001