Fig. 1

Expression of PHF20 in glioma cell lines (A172, LN229, U251, HS683 and U87) and human astrocytes cell line (HEB). a Expression of PHF20 was determined by immunoblotting (n = 3). b Relative protein levels were shown by density photometry. Error bars: ± SD. *p < 0.05, **p < 0.01 gliomas cell lines vs. HEB cell line; ^#p < 0.05 A172, LN229, U251 and HS683 cell lines vs. U87 cell line. c Boxplots for the mRNA analysis by RT-PCR (n = 6). *p < 0.05, **p < 0.01 gliomas cell lines vs. HEB cell line; ^#p < 0.05 A172, LN229, U251 and HS683 cell lines vs. U87 cell line. d mRNA levels of PHF20 were detected in U87 cells using qPCR following infection with shCON and shPHF20 (n = 3). Data presented as mean ± SD, **p < 0.01 KD group vs. NC group