Fig. 4
From: miR-422a inhibits cell proliferation in colorectal cancer by targeting AKT1 and MAPK1
MiR-422a prohibits cell cycle progression by inhibiting the activity of Raf/MEK/ERK and PI3K/AKT signaling pathways. a, b Flow-cytometry analyses of the cell cycle of the indicated CRC cells synchronized in the G2/M phase by treatment with 0.1 µM colchicine for 12 h. Error bars represent mean ± SD from three independent experiments. c, e Real-time PCR analysis of CyclinD1, p21 and p27 in the indicated cells. d, f Western blot analysis of GSK-3β, p-GSK-3β, CyclinD1, p21 and p27 in the indicated cells