Fig. 6

Treatment with AVN-C causes breast cancer cells to stain positive for annexin V. a Graphical representation of the percentage of cells in each quadrant after staining with PI and annexin V as done in biological replicate for 48-h treatment. b Representative sample of flow cytometric analysis of apoptosis after cells were stained with PI (Comp-Fl2-A) and annexin V (Comp-FL1-A) after treatment with vehicle DMSO 0.4%, AVN-A, B, C at 400 µM, or positive control STS for 96 h. c Graphical representation of the percentage of cells in each quadrant as described in (a). Results are a biological replicate done in triplicate for 96-h treatment. * Indicates statistical significance difference from 0.4% DMSO negative control for each individual quadrant as determined by a t test at p < 0.05 level of significance, ** indicates significance level at p < 0.01, *** indicates significance at p < 0.001 level