Fig. 3

Comparative protein expression analysis of angiogenic inducers and inhibitor for low-passage poorly-differentiated human MEC cells cultured in 2D and 3D microenvironments. a Representative immunoblot images of key angiogenic inducers and inhibitor for passage 10 poorly-differentiated high-grade human MEC cells cultured in 2D and 3D microenvironments. b–d Quantitative comparison of VEGF (b), bFGF (c) and TSP-1 (d) protein expression for passage 10 poorly-differentiated MEC cells cultured in 2D and 3D. Three independent repeats, with each triplicates, were performed. 2D vs. 3D: **P < 0.01; ***P < 0.001