Fig. 1

Relationship between MGMT expression, Gli1 nuclear staining and MGMT promoter methylation status in 48 cases of surgically-resected primary GBM tissues (a, b). Representative photomicrographs showed two GBM cases with distinct Gli1 activity (c). The top two photos represent a Gli1-positive GBM with visible nuclear staining of Gli1 (left) and the same sample stained with MGMT antibody showed that the percentage of MGMT staining was far more than 5% (right). The bottom two photos represent a Gli1-negative case with invisible nuclear staining of Gli1 (left). The same sample stained with MGMT antibody showed the ratio of MGMT staining was less than 5% (200×). The scale bar on the top left panel applies to the rest of the photographs and indicates 20 μm