Fig. 7

Suppressive effect of matrine, cisplatin and the combination on VEGF/PI3K/Akt signaling pathway in EJ and T24 cells. Figures are the protein expression levels of Bax, Bcl-2, Cleaved Caspase-3, Caspase-3, p-Akt, Akt, p-PI3K, PI3K, VEGFR2, VEGF and GAPDH of EJ and T24 cells treated with 4 μM of cisplatin, 5 mM of matrine and 1 μM of cisplatin and 2 mM of matrine in combination (a, f) for 48 h. Histograms present the relative grey value of the related proteins measured by ImageJ (b–e, g–j). All data are shown as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, or ****P < 0.0001 versus the control group