Fig. 3

Notch1^C1133Y mutation enhances cell invasion and regulates EMT markers. a Images of Transwell invasion assays (left) and the quantification of the cells (right). Each data point represents the mean ± SD of data from 3 independent trials. b Quantitive analysis of a. c Western blot analysis was used to assess the expression of epithelial (E-cadherin and β-catenin) and mesenchymal (N-cadherin and Vimentin) markers in Notch1^C1133Y- or Notch1^WT-transfected cell lines (HN6 and CAL27). d, e The greyscale analysis of panel C. f Real-time qPCR was used to detect the expression of classic EMT-inducer Snail (SNAI1, SNAI2) mRNA in Notch1^C1133Y- or Notch1^WT-transfected cell lines (HN6 and CAL27). g Western blot analysis was performed to assess the expression of Snail (SNAI1, SNAI2) protein after transfections. h The greyscale analysis of panel G