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Fig. 2 | Cancer Cell International

Fig. 2

From: MiR-20a-5p suppresses tumor proliferation by targeting autophagy-related gene 7 in neuroblastoma

Fig. 2

ATG7 was a direct target of miR-20a-5p in SH-SY5Y cells. a Schematic putative target sites of miR-20a-5p in 3′-UTR of ATG7, and the sequence of miR-20a-5p mutant (performed as mut). b Luciferase assay of wild type (WT) or mutated (Mut) ATG7 3′-UTR reporter co-transfected with miR-20a-5p or the control for 48 h. c Western blotting analysis of ATG7 protein levels in SH-SY5Y cells treated with miR-20a-5p mimics at indicated time and d concentration. The relative densitometric analysis of the protein bands were performed. *p < 0.05 vs. control. Data are expressed as mean ± SD, n = 3. miR-NC, negative control microRNA

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