Fig. 3

miR-200c-3p enhanced LC3-II expression in PC-3 cells. PC-3 cells were transfected with control, miR-200c-3p mimic (200 nM), or miR-200c-3p inhibitor (100 nM). At 48 h post-transfection of miR-200c-3p mimic a or miR-200c-3p inhibitor b, LC3-II and Beclin mRNA levels were determined by RT-qPCR. Levels of GAPDH were used for normalization. Data are presented as the mean ± SEM of triplicate samples. **p < 0.01 and *p < 0.05. c LC3-II expression was determined by western blotting in miR-200c-3p mimic- or inhibitor-transfected PC-3 cells in the presence or absence of NH₄Cl. d Western blotting was performed following transfection with miR-200c-3p mimic or inhibitor to determine the levels of Beclin and β-actin