Fig. 1

Effect of SHP-2 on apoptosis induced by Oxaliplatin and 5-FU in cervical cancer cells. a SHP-2 expression were detected by western blot in Hela cells and C33A cells. SHP-2 expression were detected by western blot in Hela cells, which were transfected SHP-2 shRNA1 or SHP-2 shRNA2 (b), and in C33A cells, which were transfected pCMV-SHP2-HA plasmid (e). Induction of apoptosis were measured by Annexin-V/PI double-staining assay in Hela cells, which were transfected SHP-2 shRNA1 or SHP-2 shRNA2 (c, d), and C33A cells, which were transfected pCMV-SHP2-HA plasmid (f, g) and treated different concentrations of Oxaliplatin (c, f) and 5-FU (d, g). Data are presented as mean ± SD. *P < 0.05, **P < 0.01