Fig. 5

Effect of SHP-2 on autophagy activation induced by CCCP in C33A cells. a, b LC3 expression were detected by western blot. C33A cells were transfected pCMV-SHP2-HA plasmid and treated CCCP (10 μM). Data are presented as mean ± SD. **P < 0.01 compared with CCCP treatment group. c Expressing mCherry-GFP-LC3 C33A cells were observed under a confocal microscopy after pCMV-SHP2-HA plasmid transfection or CCCP (10 μM) and Baf A1 (30 nM) treatment. Representative images are shown. d The average numbers of yellow or red puncta were obtained from three countings. Data are presented as mean ± SD. **P < 0.01 compared with CCCP treatment group