Fig. 3

The KLF4 3′UTR is a target of miR-10b. a, c Upper panel, predicted duplex formation between miR-10b and 3′UTR of KLF11 or KLF4. Lower panel, diagram of the luciferase reporter plasmids: plasmid with the full length KLF11 and KLF4 3′UTR insert and plasmid with a mutant KLF11 and KLF4 3′UTR which carried a substitution of five or six nucleotides within the miR-10b binding site, respectively. b, d The relative luciferase activity in MHCC-97L cells were determined after the KLF11 and KLF4 3′UTR or mutant plasmids were co-transfected with miR-10b overexpression vector or negative control, respectively. *P < 0.05