Fig. 4

KLF4 regulates KLF11 expression in HCC cells. a Dual-luciferase reporter assay was performed by cotransfection of the KLF11 promoter wildtype fragment or mutated fragment with overexpression of KLF4. b Reporter assay in HCC cells transfected with KLF11 promoter constructs with mutations in potential binding elements for KLF4. wt, wild type; mu, mutation type. Luciferase activity was expressed as relative to that of the pGL3-basic vector (a promoter-less vector). *P < 0.05, by one-way ANOVA. c ChIP assay demonstrated KLF4 binding to the KLF11 gene promoter. d KLF4 and KLF11 protein expression in cells transfected with KLF4 overexpression vector or siRNAs