Fig. 2

Effect of p53 domain mutations on cellular proliferation. The proliferation of the transfected SK-OV-3 cells was performed using the tetrazolium salt by measuring the ability of the mitochondrial enzymes to transform this salt into formazan. Briefly, transfection was done using Attractene for 24, 48 and 72 h. The formazan concentrations were measured at t = 0 min, and then, the cells were incubated with tetrazolium salt for 45 min, and the formazan concentration was measured using an ELISA reader at 450 nm wavelength. The results are expressed by the mean ± SD. Each condition was performed in triplicate. *P < 0.05 versus the control, and **P < 0.01 versus the control