Fig. 3

Effect of p53 site mutations on apoptosis initiation. The influence of the mutations affecting the various domains of the p53 gene on apoptosis was assessed in the transfected SK-OV-3 cells. After transfection for 24 and 48 h, the cells were harvested and Annexin V was performed by flow cytometry. a, b Are examples of the results obtained by flow cytometry. a Represents 24 h and b is 48 h after the transfection using the four plasmids (ctl, p53 WT, p53 R249S and p53 P72 R for 1, 2, 3 and 4, respectively). Each experiment was performed three times, and the mean ± SD are displayed in c. *P < 0.05 versus the control