Fig. 1

PIM1-shRNA induced PIM1 gene silencing in ACC-M cells. ACC-M cells were transfected with 4 μg of shRNA expression plasmids against PIM1 (RNAi) or with shRNA-NC (vector control). RNAi and control samples were collected at 24 and 48 h and analyzed with real time-PCR (a) and western blotting for the expression of PIM1 (b). GAPDH was amplified as the real time PCR internal control, β-actin was used as the loading control for western blotting. a *Significantly different compared to control in 24 h (p < 0.05) and ^#significantly different compared to control group in 48 h (p < 0.05). Vector control: negative control for transfection of non-targeting sequence; PIM1-shRNA-1, PIM1-shRNA-2, PIM1-shRNA-3 and PIM1-shRNA-4 are small hairpin RNA targeted against PIM1