Depletion of membrane cholesterol compromised caspase-8 imparts in autophagy induction and inhibition of cell migration in cancer cells

Table 1 Details of primers used in experiment

mRNAs	NCBI gene accession	Primers	Sequences (5′–3′)	Tm	Product size (bp)
CASPASE-8	XM_005246891.4	Forward	GTTGTGTGGGGTAATGACAATCT	58.92	183
CASPASE-8	XM_005246891.4	Reverse	CCATTCCTGTCCCTAATGCTG	58.42	183
MAP1LC3A	XM_011529085.2	Forward	TCCTGAACTGAGCTGCCTCTA	60.27	131
MAP1LC3A	XM_011529085.2	Reverse	CACCCAGAGGGACAACCCTA	60.55	131
BECN-1	NM_001313998.1	Forward	GGTTGAGAAAGGCGAGACACG	61.52	133
BECN-1	NM_001313998.1	Reverse	TGTCCACTGCTCCTCAGAGT	60.18	133
RIPK-1	NM_001317061.1	Forward	TGGCAGGAAAGAAGGCCC	59.56	144
RIPK-1	NM_001317061.1	REVERSE	CCTAGGCTGCCCATGGTGTT	62.22	144
CFLAR	XM_017005196.1	Forward	GCAGCCTCTTGGAGGTGGAT	61.92	116
CFLAR	XM_017005196.1	Reverse	CGCAGTACACAGGCTCCAGA	61.88	116
ATG-3	M_001278712.1	Forward	CGAGTGAAGCAAAGCGAGGA	60.67	128
ATG-3	M_001278712.1	Reverse	GACCGGACCCAGCTGTCA	61.31	128
Cyclophylin-A	M_001300981.1	Forward	CTCGAATAAGTTTGACTTGTGTTT	56.17	165
Cyclophylin-A	M_001300981.1	Reverse	CTAGGCATGGGAGGGAACA	58.38	165
PUM-1	NM_014676.2	Forward	AGCCCAATAACAACCTGGCA	59.89	144
PUM-1	NM_014676.2	Reverse	CGCCGAGAGAACTGCTACTT	59.83	144
PIK3C3	XM_011526031.2	Forward	CCTGGAAGACCCAATGTTGAAG	59.18	236
PIK3C3	XM_011526031.2	Reverse	CGGGACCATACACATCCCA	58.78	236

ISSN: 1475-2867