Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: Catalase down-regulation in cancer cells exposed to arsenic trioxide is involved in their increased sensitivity to a pro-oxidant treatment

Fig. 2

Catalase gene silencing and catalase activity inhibition sensitized Resox cells to ascorbate/menadione treatment. a Efficiency of catalase gene silencing (siRNA) in breast cancer cell lines was evaluated by western blot. b, c MCF-7 cells were transfected 48 h with control (siRNA CTL) or catalase siRNA (siRNA CAT). Then, cells were incubated with various concentrations of H2O2 (mM) or with indicated menadione at μM concentrations associated with ascorbate in a ratio Asc/Men 1:100. Cytotoxicity was evaluated by MTT. d, e Resox cells were transfected 48 h with control (siRNA CTL) or catalase (siRNA CAT) siRNA. Then, cells were incubated as reported in B-C. f, g MCF-7 cells were incubated for 24 h with PBS or 5 mM ATA. Then, cells were incubated as reported in (bc). hi Resox cells were incubated for 24 h with PBS or 5 mM ATA. Then, cells were incubated as reported in (b, c). Data are mean ± s.e.m. Groups were compared using two-way ANOVA test for (bi). *p value < 0.05; **p value < 0.01

Back to article page