Fig. 4

Cell viability analysis of H295R cell line treated with everolimus (Eve) alone or with mitotane (Mit). MTS assay in monolayer cells after everolimus treatment for 3 days (a) and (b) a combination of Eve and 10 μM Mit for 72 h; (c) MTS assay in spheroids after treatment with Eve or a combination of Eve and Mit; d phase-contrast photomicrography of spheroids after 72 h of treatment; e Confocal microscopy images of spheroids after 72 h of treatment (100 μm). The results are expressed as the mean ± SEM; ANOVA **p < 0.01; ***p < 0.001; ****p < 0.0001; n = 3