Table 1 Cell culture media preparations
From: Characterization of MCF-12A cell phenotype, response to estrogens, and growth in 3D
Name | Base media | Serum | Additives |
|---|---|---|---|
MCF-12A/MCF-10A | DMEM/F12, 1:1 | 5% equine | 20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 0.1 μg/mL cholera toxin, 10 μg/mL insulin |
MCF7 | DMEM | 5% fetal bovine | |
Rinse | DMEM/F12, 1:1, phenol red-free | ||
CD-FBS | DMEM/F12, 1:1, phenol red-free | 5% charcoal–dextran stripped fetal bovine | |
CD-ES | DMEM/F12, 1:1, phenol red-free | 5% charcoal–dextran stripped equine | 20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 0.1 μg/mL cholera toxin, 10 μg/mL insulin |