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Table 1 Cell culture media preparations

From: Characterization of MCF-12A cell phenotype, response to estrogens, and growth in 3D

Name Base media Serum Additives
MCF-12A/MCF-10A DMEM/F12, 1:1 5% equine 20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 0.1 μg/mL cholera toxin, 10 μg/mL insulin
MCF7 DMEM 5% fetal bovine  
Rinse DMEM/F12, 1:1, phenol red-free   
CD-FBS DMEM/F12, 1:1, phenol red-free 5% charcoal–dextran stripped fetal bovine  
CD-ES DMEM/F12, 1:1, phenol red-free 5% charcoal–dextran stripped equine 20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 0.1 μg/mL cholera toxin, 10 μg/mL insulin