Fig. 4

The negative RUNX3 regulation by miR-544. a The potential binding sites of miR-544 and RUNX3 predicted by bioinformatical analysis. b The relative luciferase activity in NK-92 co-transfected with miR-544 inhibitor or NC together with WT or Mut vector plasmids of RUNX3. The expression of RUNX3 at mRNA and protein levels in NK-92 cells transfected with miR-544 inhibitor or NC. *Refers to compare with the NC group, P < 0.05. c The relative luciferase activity in NK-92 co-transfected with miR-544 mimic or pre-NC together with WT or Mut vector plasmids of RUNX3. The expression of RUNX3 at mRNA and protein levels in NK-92 cells transfected with miR-544 mimic or pre-NC. d The mRNA expression levels of miR-544 in NK-92 cells co-transfected with miR-544 mimic or pre-NC. *Refers to compare with the pre-NC group, P < 0.05