Fig. 3
From: Autophagy inhibition improves the cytotoxic effects of receptor tyrosine kinase inhibitors
Single versus combined treatment with RTKi and autophagy blocking agents. RTKi were used as a single treatment or in combination with a Chloroquine (CQ; 25 μM) or b Spautin-1 (SP1; 10 μM). Changes in LC3, BECLIN 1, p62 and ATG5 protein levels were evaluated by western blot. Changes in autophagy and apoptosis activation were validated in parallel. Apoptosis was evaluated by measuring intensity of PARP cleavage. Besides, MCL1 protein expression was checked out in order to determine the capacity of combined treatment to decrease it. GAPDH served as a loading control protein. D DMSO, CQ Chloroquine, SP1 Spautin-1, Af Afatinib (8 μM), Sor Sorafenib (14 μM), TP TP-0903 (0.15 μM)