Fig. 5From: Autophagy inhibition improves the cytotoxic effects of receptor tyrosine kinase inhibitorsAutophagic vacuoles creation. Autophagy activation upon treatment with Afatinib was additionally validated using immunofluorescence in order to visualize a creation of autophagy vacuoles; ×60 magnification. Green—autophagy vacuoles; Blue—Hoechst, nuclear staining. The combined treatment with Afatinib and a CQ or b SP1 was usedBack to article page