Fig. 2

miR-365a-3p promotes the proliferation, migration, and invasion ability of lung cancer cells. a Plate clone formation efficiencies of A549 or SPC-A-1 cells following overexpression of miR-365a-3p by agomiR or inhibition of miR-365a-3p by antagomiR treatment, as compared with controls. b Representative images of A549 or SPC-A-1 cells stained with Hoechst (blue) and EdU (red), which indicates DNA synthesis, after transfection with agomiR-365, antagomiR-365, or controls. c Wound healing assay in A549 or SPC-A-1 cells transfected with agomiR-365, antagomiR-365, or controls. d Transwell migration and invasion assay in A549 or SPC-A-1 cells transfected with agomiR-365, antagomiR-365, or controls. For all experiments, agomiR-NC and antagomiR-NC were used as the negative controls for agomiR-365 and antagomir-365, respectively. Left panel, representative images. Right panel, quantitative data. All experiments were performed in triplicate and repeated three times independently. *P < 0.05, **P < 0.01, ***P < 0.001 compared to controls