Fig. 5

Chemical inhibition of LSD1 reduces PSA expression driven by AR-V7. a Western blot analysis of AR, LSD1 and PSA expression in LNCaP cells transfected with either the AR-WT or the AR-V7 splice variant as indicated. LNCaP cells were incubated in CSS-media for 24 h prior to transfection and after 24 h LSD1-C76 (250 μM) or DMSO solvent control were added for 18 h. b Quantification of PSA expression in three independent experiments. PSA levels were normalized against the DMSO treated samples. P values were derived using an unpaired t test with Welch’s correction. ***P < 0.05