Fig. 4

Identification of VASP as the molecular target of miR-4455. a Schematic of wild-type and mutant-type 3′-UTRs of the VASP plasmids, and relative luciferase activity assays of luciferase reporter plasmids containing VASP-wt or VASP-mut 3′-UTR performed in MGC-803 cancer cells. c The mRNA levels of VASP in MGC-803 cells cells transfected with miR-4455 mimic, miR-4455 inhibitor or miR-NC, as determined by qRT-PCR. Beta-actin served as an internal control. b, d Protein levels of VASP in MGC-803 cells transfected with miR-4455 mimic, miR-4455 inhibitor or miR-NC, as determined by Western-blot analysis. Beta-actin served as an internal control. **P < 0.01, vs. miR-NC group