Fig. 2

The expression of PAR1, 2, and 4 in different groups of TE-1 cells measured using qPCR and WB. a–c The protein expression of PARs in TE-1 cells with no-insert CRISPR/Cas9 plasmid vehicle control (CRISPR-N), with no-insert pIRES2 plasmid vehicle control (pIRES2-N), with CRISPR/Cas9 knockdown plasmid (CRISPR-PAR), and with expression plasmid (pIRES2-PAR); d the relative mRNA expression of PARs in each group relative to GAPDH in each vehicle control (P < 0.05, n = 3)