Fig. 6

Effects of IFNγ and Smac mimetics on cell death in other NSCLC cells and HAECs. a, b H1437 cells were incubated with 10 ng/ml IFNγ or 250 ng/ml poly(I:C) plus different doses of AZD5582 (a) or with 10 ng/ml IFNγ alone or plus 20 nM AZD5582, 20 nM SM164, or 250 nM BV6 (b) for 60 h, and cell viabilities were assessed. c H1437 cells were treated with IFNγ (10 ng/ml) or poly(I:C) (250 ng/ml) plus different doses of AZD5582 for 60 h, and the detached cells were collected and counted with trypan blue by a TC20 automated cell counter (n = 3). d H1437 cells were treated with IFNγ (10 ng/ml) and AZD5582 (20 nM) for 27 h, and cell lysates at equal amounts were subjected to Western blotting with indicated antibodies. e–h A549, Calu-3 and H441 NSCLC cell lines or HAECs were treated with IFNγ (25 ng/ml) or poly(I:C) (250 ng/ml) or with the indicated amounts of IFNγ (h) in the presence of DMSO or 20 nM of AZD5582 or SM164 for 72 h. Cell viabilities (a–h) were assessed by MTS assay and cell survival rates were calculated by comparison to DMSO-treated control cells (n = 3). **p < 0.01, ***p < 0.001 versus DMSO. Results represent three independent experiments