Fig. 2

Establishment of a SPC-A1 cell line expressing low levels of RelB. a RelB mRNA expression of the established cell lines as determined by PCR analysis and normalized to β-actin gene expression. b Protein levels of RelB expression in the SPC-A1-shctrl and SPC-A1-shRelB cell lines were determined by Western blotting. The level of each protein was normalized against β-actin. c RelB silencing affects the expression of other NF-κB subunits. Western blot analysis of individual NF-κB family members protein expression. Protein expression in the whole portion was normalized against β-actin