Fig. 5

SEMA5A-induced VEGF-C expression is mediated through Met. a Western blot was used to determine VEGF-C protein expression in HeLa, Siha, and Caski cells treated with 100 ng/mL SEMA5A in the absence (−) or presence (+) of the Met inhibitor SU11274 (2.5 μM) or a Met neutralizing antibody (met antibody; 2 μg/mL). Tubulin was used as an internal loading control. Met inhibition suppressed the enhancement of VEGF-C expression by SEMA5A. b ELISA measurement of VEGF-C secretion was determined in HeLa, Siha, and Caski cells treated with 100 ng/mL SEMA5A in the absence (−) or presence (+) of SU11274 or Met antibody. VEGF-C ELISA results were consistent with the western blot results. Data shown are representative of at least three independent experiments. *P < 0.05 compared with basal level or SEMA5A alone (b). SEMA5A semaphorin 5A, VEGF-C vascular endothelial growth factor-C, ELISA enzyme-linked immunosorbent assay