Fig. 3

Compound-1H induces cell cycle arrest at S-phase to suppress glioblastoma cell proliferation. a Proliferation and quantification of U87 and LN229 cells were determined by BrdU staining assay with the treatment of compound-1H for 48 h. Scale bar, 100 μm. b The cell cycle of U87 and LN229 cells was measured by flow cytometry in the presence of vehicle or compound-1H, and the percentages of cell population in different periods were quantitated with three independent experiments. c Effects of compound-1H on the expression levels of cyclin A, cyclin E, P21 and P53 in U87 and LN229 cells were determined by western blot, with β-tubulin as a loading control. All data were demonstrated as the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001