Fig. 6

Colony formation of T98G cells in a fibroblast layer in the presence of different concentrations of carnosine. T98G cells (2500) were placed inside the cloning ring and fibroblasts (P0375; 50.000) outside of it. The ring was removed 4 h later and after 4 weeks of cultivation in the presence of different concentrations of carnosine the cultures were fixated and stained with DAPI and a TE-7 specific antibody. Fluorescence images are presented in the upper part of panel a and pictures generated by ImageJ for the determination of area occupancy and the number of colonies formed is presented in the lower part. On the right side of the graph, area occupancy (b 100% equals the whole well area) and the number of tumor cell colonies formed (c) is presented. Statistical significance using data obtained from 8 to 10 independently treated cultures for each concentration of carnosine was determined using Student’s t-test with: *p < 0.05; ***p < 0.0005