Fig. 4

Enhanced LDH release in MDA-MB-231 cells treated with As^III combined with Tetra. After treatment with 10 μM As^III and 4 μg/ml Tetra, alone or in combination, in the presence or absence of 0.25 μM wortmannin, for 48 h, LDH leakage was measured using the LDH-Cytotoxic test kit as described under “Materials and methods”. Significant difference between control and treatment with As^III and Tetra, alone or in combination, are shown (^†p < 0.0001 vs. control; ^∫p < 0.0001 vs. As^III alone; ^♪p < 0.001 vs. Tetra). As, As^III; Tetra, tetrandrine